Journal: Cells
Article Title: The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection
doi: 10.3390/cells10030645
Figure Lengend Snippet: Effect of a ptpB deletion on the NO˙ resistance of S. aureus SA564. Cells of S. aureus strain SA564 (filled symbols) and its isogenic Δ ptpB mutant (opened symbols) were cultured in presence and absence of 12.5 µM DEA NONOate in TSB under static conditions at 37 °C and 5% CO 2 for 12 h. Growth was measured by optical density using the oCelloScope BCA algorithm. ( a ) Growth kinetics of DEA NONOate treated (green symbols) and untreated (black symbols) cell suspensions. The graph represents the average growth values ± SD of four biological experiments. ( b ) Relative growth rates of DEA NONOate treated cells in relation to the growth rates recorded for the untreated controls at 12 h of growth. * p < 0.05 (Mann-Whitney U test).
Article Snippet: Growth of the cultures was monitored for 12 h using digital time-lapse microscopy with the oCelloScope instrument (BioSense Solutions, Farum, Denmark) under static conditions at 37 °C and 5% CO 2 .
Techniques: Mutagenesis, Cell Culture, MANN-WHITNEY